Composite

Part:BBa_K2960012

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-20)


mlrA microcystinase

This is a composite part consisting of a constitutively-active promoter sequence (J23100), a ribosome binding site (BBa_J61100), the mlrA gene (BBa_K1378001), a 3x FLAG tag (BBa_K823034), and a terminator (BBa_B0015).

MlrA is the first enzyme in the microcystin-degradation pathway. It is a microcystinase that degrades cyclic microcystin-LR to form a linear intermediate. To do so, mlrA hydrolyzes the peptide bond between Adda at position 5 and the amino acid residue at position 4.

We have also included a 3X FLAG tag at the end of the sequence. This permits protein expression to be detected, quantified and/or purified by western blot, SDS-PAGE, and other methods.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 420
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None